DKK4 (Dickkopf-related Protein 4, Dickkopf-4, Dkk-4, hDkk-4), Goat
Biozol Catalog Number:
USB-D3795-20D
Supplier Catalog Number:
D3795-20D
Alternative Catalog Number:
USB-D3795-20D-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Synthetic peptide corresponding to RQHARLRVCQKIEKL, from human DKK4, at C-terminal (NP_055235.1).
DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50-55aa in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12aa. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 1-3ug/ml, Jurkat lysate observed on ~30-35kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
