DNA Marker Lambda EcoRI, HindIII, 13 Fragments (125-21226bp), BioGenomics(TM)

Product Overview

• Article Name: DNA Marker Lambda EcoRI, HindIII, 13 Fragments (125-21226bp), BioGenomics(TM)
• Biozol Catalog Number: USB-D3930-23
• Supplier Catalog Number: D3930-23
• Alternative Catalog Number: USB-D3930-23-5X50,USB-D3930-23-25X50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Lambda DNA was completely digested by both EcoRI and HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125. The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp (indicated*) may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65C for 5min and then cooling on ice for 3min. Concentration: ~0.5mg DNA/ml Form: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 1mM EDTA. 6X Loading Dye Solution L3350: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA. Prepare DNA Marker as follows: 1ul (0.5ug) DNA marker 1ul of 6X Loading Dye Solution 4ul ddH2O 1. Vortex gently prior to use 2. Heat 5 min. 65C, cool on ice 3 min. 3. Apply 6ul DNA marker per 5mm of agarose gel lane width. 4. Visualize bands with ethidium bromide staining. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.