DNA Polymerase I Klenow Fragment, CAS [[9012-90-2]]

Product Overview

• Article Name: DNA Polymerase I Klenow Fragment, CAS [[9012-90-2]]
• Biozol Catalog Number: USB-D3940
• Supplier Catalog Number: D3940
• Alternative Catalog Number: USB-D3940-300, USB-D3940-1500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

DNA Polymerase I Large Fragment exhibits the 5 ---> 3 polymerase and 3 ---> 5 exonuclease activity of DNA Polymerase. Lacks 5 ---> 3 exonuclease activity. Applications: Random primer labeling Sanger sequencing cDNA Second Strand Synthesis 3 end-labeling, filling Blunting of DNA ends: fill-in 5-overhangs or/and removal of 3-overhangs, see protocol Oligonucleotide-directed site-specific mutagenesis. Source: E. coli cells with a cloned fragment of the polA gene Storage Buffer: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 0.1mM EDTA, 1mM DTT, 50% glycerol. Unit Definition: 1u of DNA Polymerase I Klenow Fragment catalyzes the incorporation of 10nmoles of deoxynucleotides into polynucleotide fraction in 30 minutes at 37C. Reaction Buffer (Supplied): D3940A DNA Polymerase I Klenow Fragment, Reaction Buffer, 10X: Supplied as a liquid in 500mM Tris-HCl, pH 8.0, 50mM magnesium chloride, 10mM DTT Inhibition and Inactivation: Inhibitors: metal chelators, nucleotide analogs PPi, Proprietray information (at high concentrations). Inactivated by heating at 75C for 10 min or by addition of EDTA. Quality Control: Endodeoxyribonuclease Assay: No detectable degradation was observed after incubation of supercoiled plasmid DNA with DNA Polymerase I Klenow Fragment. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 62
• CAS Number: [9012-90-2]