This is an N-terminal truncation of the E. coli DNA Polymerase I protein, at amino acid 323. This truncation results in the deletion of the 5 to 3 exonuclease domain, but leaves the polymerase function unaffected. The introduction of two additional mutations (D355A and E357A) results in the abolition of the 3 to 5 exonuclease activity. Source: E. coli Unit Definition: 1u catalyzes the incorporation of 10nmoles of deoxynucleotides into polynucleotide fraction in 30 minutes at 37C. Reaction Buffer (Supplied): D3941-01A: DNA Polymerase I Klenow Fragment, Reaction Buffer, 10X: Supplied as a liquid in 200mM Tris, pH 7.5, 50mM MgCl2, 20mM DTT. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Form:
Supplied as a liquid in 50mM KPO4, pH 7.0, 100mM KCl, 1mM DTT, 50% glycerol.
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