DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. DnaK (residues 508-638) of the substrate binding domain is alpha helical and appears to act as a lid covering the substrate binding cleft. Additional amino acid (Met) is attached at the N-terminus. Molecular Weight: 14.6kD (132 amino acids) Sequence: MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIESALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDVVDAEFEEVKDKK Biological Activity: Not determined Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
14.6
Purity:
95% by SDS PAGE, Purified by using conventional chromatography techniques.
Form:
Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 1mM DTT, 10% glycerol.
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