Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. Directions per Liter: Mix 1.15 grams of drop-out mix with 26.7g drop-out base w/ carbohydrate (YNB) (or 6.7g drop-out base w/o carbohydrate) (or 43.7g drop-out base w/ agar) per liter of distilled/deionized water. Heat with stirring until completely solubilized. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. added: Directions per Liter (Plates): Mix 1.15 grams of drop-out mix with 43.7g D9510-01 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). -SKfor making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Appearance: White, homogeneous, free flowing powder Solubility: Colorless, clear, complete pH: As Reported Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder) Y2025: Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder) Y2030: Yeast Nitrogen Base w/o AA, Carbohydrate & w/o AS (YNB) (Powder) Y2035: Yeast Nitrogen Base w/AA & w/AS, Vitamin Free (YNB) (Powder) Y2035-05: Yeast Nitrogen Base (YNB) w/AA & w/AS, Vitamin Free, w/o Dextrose (Powder) Y2036: Yeast Nitrogen Base w/o AA, AS, Thiamine HCl (Powder) Y2037: Yeast Nitrogen Base w/o AA & w/AS (YNB) and w/o Fe, Zn, Mn, Cu (Powder)
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