DUSP3, Recombinant, Human, aa1-185, His-Tag (Dual Specificity Protein Phosphatase 3, Dual Specificity Protein Phosphatase VHR, Vaccinia H1-related Phosphatase, VHR)
Biozol Catalog Number:
USB-D9840-06M
Supplier Catalog Number:
D9840-06M
Alternative Catalog Number:
USB-D9840-06M-100,USB-D9840-06M-500
Manufacturer:
US Biological
Category:
Molekularbiologie
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Source: Recombinant corresponding to aa1-185 from human DUSP, fused to His-tag at N-terminal expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP Molecular Weight: ~22.6kD (MALDI-TOF) Biological Activity: Determined by measuring the amount of enzyme hydrolyzing 1nm of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH 7.5 using 10mM of substrate, corresponding to a specific activity of >1750IU/mg. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
