Eco52I (XmaIII)

Product Overview

• Article Name: Eco52I (XmaIII)
• Biozol Catalog Number: USB-E0374-25
• Supplier Catalog Number: E0374-25
• Alternative Catalog Number: USB-E0374-25-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

5-C G G C C G-3 3-G C C G G C-5 Sequence: 5-C G G C C G-3 3-G C C G G C-5 Source: Escherichia coli RFL52 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Eco52I required to digest 1ug of lambda DNA-Eco81I fragments in 1 hour at 37C in 50ul of recommended reaction buffer. Form: Supplied as a liquid in 10mM Tris-HCl, pH 8.2, 500mM sodium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-60: Restriction Enzyme Buffer, 10X: Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5, 3mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20 minutes. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Enzyme Properties: Methylation Effects: Dam, Never overlaps - no effect Dcm: Never overlaps - no effect CpG: Completely overlaps - blocked EcoKI: Never overlaps - no effect EcoBI: Never overlaps - no effect Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 2 PhiX174: 0 pBR322: 1 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0 Compatible Ends: Bsp120I, CfrI, NotI Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion of Eco52I (10u/ug lambda DNA x 16 hours). Ligation/Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed during incubation with 10 units of Eco52I for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.