Endonuclease III, Recombinant, E. coli, aa1-211, His-Tag (nth, b1633, JW1625, DNA-(apurinic or apyrimidinic site) Lyase)
Biozol Catalog Number:
USB-E2291-41C
Supplier Catalog Number:
E2291-41C
Alternative Catalog Number:
USB-E2291-41C-250
Manufacturer:
US Biological
Category:
Molekularbiologie
Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5 from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Description: Recombinant E. coli NTH (Endonuclease III) protein fused to His-tag at the N-terminus, expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I Molecular Weight: 25.7kD (231aa) confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C or lower. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
