EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Recombinant rat EGF is a 6.2kD globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds. Biological Activity: The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1ng/ml, corresponding to a specific activity of >1x10e7 units/mg. Amino Acid Sequence: MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ml (1EU/ug)
Form:
Supplied as a lyophilized powder. Reconstitute with sterile ddH2O, carrier protein to a final concentration of 0.1-1mg/ml. Do not vortex.
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