Epidermal Growth Factor Receptor, T790M, L858R, Recombinant, Human, GST-Tag

Product Overview

• Article Name: Epidermal Growth Factor Receptor, T790M, L858R, Recombinant, Human, GST-Tag
• Biozol Catalog Number: USB-E3375-47E
• Supplier Catalog Number: E3375-47E
• Alternative Catalog Number: USB-E3375-47E-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

The L858R mutation has been associated with non-small cell lung cancer patients who demonstrate clinical responsiveness to the tyrosine kinase inhibitor gefitinib (Iressa, ZD1839). Source: Recombinant human EGFR T790M L858R, aa668-1210, with N-terminal GST tag, expressed in baculovirus expression system. Molecular Weight: ~88kD Specific Activity: As reported Reaction Buffer: 50mM HEPES (pH7.5), 10mM MgCl2, 1mM EDTA, 0.01% BRIJ- 35 and 200uM of ATP. Substrate: 4uM Tyr peptide 4 from Invitrogen. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: The enzyme reaction was carried out for 1h at room temperature in reaction buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Product Properties

• Molecular Weight: 88
• NCBI: 005228
• UniProt: P00533
• Purity: 80%
• Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM NaCl, 0.05% Tween 20, 50% glycerol, 3mM DTT.