Eukaryotic Translation Initiation Factor 4E, aa1-217, Recombinant, Human (eIF4E, eIF4EL1)
Biozol Catalog Number:
USB-E8949-44
Supplier Catalog Number:
E8949-44
Alternative Catalog Number:
USB-E8949-44-50,USB-E8949-44-250
Manufacturer:
US Biological
Category:
Molekularbiologie
EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Source: Recombinant corresponding to aa1-217 of human recombinant EIF4E protein, fused to His-tag at N-terminus, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
