Human Factor XI is a plasma glycoprotein which circulates in a non-covalent complex with high molecular weight kininogen. The mature molecule is synthesized in the liver. It is a two-chain homodimer with a molecular weight of ~160kD. It is estimated that 5% of the total mass is attributable to carbohydrate. The two identical monomers have molecular weights of 80kD. They are joined together by disulfide bonds. By SDS-PAGE analysis, factor XI appears as a single band both non-reduced (Mr 160kD) and reduced (Mr 80kD). Applications: Suitable for use as an inhibitor in clotting assays, Western Blot, RIA and ELISA. Useful for purification of Factor XI. Other applications have not been tested. Recommended Dilutions: Western Blot: Non-reduced samples only Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[2B32]
Purity:
Purified by ammonium sulfate precipitation, ion exchange chromatography and gel filtratation from ascites.
Form:
Supplied as a liquid, 50% glycerol. No preservative added.
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