Human Factor XI is a plasma glycoprotein which circulates in a non-covalent complex with high molecular weight kininogen. The mature molecule is synthesized in the liver. It is a two-chain homodimer with a molecular weight of ~160kD. It is estimated that 5% of the total mass is attributable to carbohydrate. The two identical monomers have molecular weights of 80kD. They are joined together by disulfide bonds. By SDS-PAGE analysis, factor XI appears as a single band both non-reduced (Mr 160kD) and reduced (Mr 80kD). Applications: Suitable for use as an inhibitor in clotting assays, Western Blot, RIA and FLISA. Useful for purification of Factor XI. Other applications have not been tested. Recommended Dilutions: Western Blot: Non-reduced samples only Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[2B32]
Purity:
Purified by ammonium sulfate precipitation, ion exchange chromatography and gel filtratation from ascites.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
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