FABP5 is a member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to specifically bind fatty acids (FAs) with high affinity for stearic and linoleic acids. FABP5 is expressed in endothelial cells of the microvasculature of the placenta, heart, skeletal muscle, small intestine, lung, and renal medulla. FABP4 and FABP5 are closely related and both are expressed in adipocytes. Absence of FABP5 resulted in increased systemic insulin sensitivity in two models of obesity and insulin resistance. Recombinant human FABP5, aa1-135, was expressed in E. coli and purified by conventional chromatography. AA Sequence: MATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI YEKVE Applications: Suitable for use in Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
