FCRLB, Recombinant, Human (FCRL2, FCRY, FREB2, Fc Receptor-like B, Fc Receptor homolog expressed in B cells Protein 2, Fc Receptor-like and Mucin-like Protein 2, Fc Receptor-like Protein 2, Fc Receptor-related Protein Y, FCRLM2, F
FCRLB, Recombinant, Human (FCRL2, FCRY, FREB2, Fc Receptor-like B, Fc Receptor homolog expressed in B cells Protein 2, Fc Receptor-like and Mucin-like Protein 2, Fc Receptor-like Protein 2, Fc Receptor-related Protein Y, FCRLM2, F
FCRLB, Recombinant, Human (FCRL2, FCRY, FREB2, Fc Receptor-like B, Fc Receptor homolog expressed in B cells Protein 2, Fc Receptor-like and Mucin-like Protein 2, Fc Receptor-like Protein 2, Fc Receptor-related Protein Y, FCRLM2, F
Biozol Catalog Number:
USB-F0025-73
Supplier Catalog Number:
F0025-73
Alternative Catalog Number:
USB-F0025-73-50
Manufacturer:
US Biological
Category:
Molekularbiologie
FCRLB, also known as FCRL2, FCRY, and FREB2, is a 60kD protein with sequence homology to classical Fc receptors. There are at least six type 1 transmembrane FCRL proteins and two that lack transmembrane segments. Each family member contains between three and nine immunoglobulin-like domains. FCRL proteins are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation. According to our testing, FCRLB binds to purified human IgG with high affinity. Human FCRLB shares 82% and 85% aa sequence identity with mouse and rat FCRLB, respectively. It contains a putative signal peptide, three immunoglobulin-like domains, and a mucin-like stalk that is rich in Pro, Ser, and Thr residues. The stalk region also contains di-Leu motifs and an unpaired cysteine. Alternative splicing generates isoforms with deletions in the putative signal peptide and substitutions and/or truncations in the third Ig -like domain. When expressed in transfectants, FCRLB is not secreted but shows a diffuse intracellular localization. FCRLB is expressed at a low level in placenta and in B lineage cells of the germinal center. It is upregulated in B cells by BAFF and LPS. Among non-hematopoietic cells, FCRLB is expressed in fibroblasts, melanocytes, and melanoma. It is preferentially expressed in nonproliferating cells and at the onset of apoptosis. Source: Murine myeloma cell line, NS0-derived. Ala18-Ser426, with a C-terminal 6x-His tag. Molecular Weight: 65-70kD (SDA-PAGE, reducing conditions) Biological Activity: Measured by the ability to bind human IgG with an estimated KD <120nM. Endotoxin: 1EU/ug (LAL) Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
