Ferredoxin 1, Recombinant Human, aa61-184, T7-Tag (Adrenodoxin, Adrenal ferredoxin, Hepatoredoxin, ADX, FDX, LOH11CR1D)

Catalog Number: USB-F3979
Article Name: Ferredoxin 1, Recombinant Human, aa61-184, T7-Tag (Adrenodoxin, Adrenal ferredoxin, Hepatoredoxin, ADX, FDX, LOH11CR1D)
Biozol Catalog Number: USB-F3979
Supplier Catalog Number: F3979
Alternative Catalog Number: USB-F3979-100,USB-F3979-500
Manufacturer: US Biological
Category: Molekularbiologie
Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Source: Recombinant protein corresponding to aa61-184 from human Ferredoxin 1. fused to T7-Tag at N-terminal,, expressed in E. coli. Swiss/UniProt Accession: P10109. Molecular Weight: ~15kD (139aa) confirmed by MALDI-TOF Amino Acid Sequence: SSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 15
NCBI: 004100
UniProt: P10109
Purity: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL)
Form: Supplied as liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.