Ferritin, Liver, Human, Western Blot Control (Liquid) for F4015-17D

Article Name:	Ferritin, Liver, Human, Western Blot Control (Liquid) for F4015-17D
Biozol Catalog Number:	USB-F4015-17L
Supplier Catalog Number:	F4015-17L
Alternative Catalog Number:	USB-F4015-17L-100
Manufacturer:	US Biological
Category:	Molekularbiologie
Application:	WB

Western Blot Control for F4015-17D. Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. Natural iron is quite insoluble. Excess iron is harmful. It can catalyze the formation of potentially damaging reactive oxygen species. Cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels. The major pool of body iron (~85%, 40-50mg/kg) is found in circulating hemoglobin and muscle myoglobin. Iron absorption occurs primarily in the intestine (duodenum) and inversely related to body iron reserve. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), iron regulatory proteins (IRPs), etc. play a key role in iron metabolism. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Its three dimensional structure is conserved in all species despite very low sequence identity from bacteria to human. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species, the heavy subunit (~21kD, FTH) and the light subunit (~ 19kD, FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains, where H-type is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3. FTH subunit (rich in human heart) is located on chromosome 11. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H:L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy. Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation. L-chains assist in core formation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane. Ready to use. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Purity:	Highly purified ( 95%).
Form:	Supplied as a liquid in denaturing SDS-PAGE sample buffer