Acidic FGF is a mitogen for cells of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, astrocytes, neuroblasts, osteoblasts, and smooth muscle cells. This protein is composed of 140aa residues, and has a 55% homology with basic FGF, including two conserved cysteine residues. Biological Activity: Human acidic FGF is mitogenic for a variety of normal cells in culture including fibroblasts, glial cells, vascular endothelial cells. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
15.5
Purity:
Purified >95% pure by HPLC analysis and SDS gel electrophoresis.
Form:
Supplied as a liquid in 10mM acetic acid, pH 3.1. Unstable without a carrier protein like gelatin or bovine serum albumin. It is recommended to add some of these proteins, prior to aliquoting, for longer storage.
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