Acidic Fibroblast Growth Factor, Recombinant, Human (aFGF)

Product Overview

• Article Name: Acidic Fibroblast Growth Factor, Recombinant, Human (aFGF)
• Biozol Catalog Number: USB-F4210-10D
• Supplier Catalog Number: F4210-10D
• Alternative Catalog Number: USB-F4210-10D-100,USB-F4210-10D-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Acidic fibroblast growth factor(aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography. Molecular Weight: 15.9kD (141aa) Amino Acid Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D Biological Activity: The ED50 for this effect is < 2.9 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 340,000 units/ml). Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 15.9
• Purity: 95% by SDS PAGE
• Form: Supplied as a liquid in 20mM Tris, pH 7.0, 100mM sodium chloride.