Fibroblast Growth Factor 4, Recombinant, Human (FGF-4, Heparin Secretory Transforming Protein 1, HSTF-1, HST-1, HST, Transforming Protein KS3, Heparin-binding Growth Factor 4, HBGF-4, HSTF1, KS3)
Biozol Catalog Number:
USB-F4210-50A
Supplier Catalog Number:
F4210-50A
Alternative Catalog Number:
USB-F4210-50A-5,USB-F4210-50A-25
Manufacturer:
US Biological
Category:
Molekularbiologie
FGF-4 is a heparin-binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-4 signals through FGFR 1c, 2c, 3c, and 4. Recombinant Human FGF-4 is a 19.7kD protein consisting of 182 amino acid residues. Recombinant corresponding to 182aa from human Fibroblast Growth Factor 4 expressed in E. coli Molecular Weight: ~19.7kD Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is 10 ng/ml, corresponding to a specific activity of 1x10e5 units/mg. Amino Acid Sequence: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Further dilute for extended storage with a buffer containing a carrier protein (0.1% BSA or HSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.4, 100mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex. Further dilute for extended storage with a buffer containing a carrier protein (0.
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