Fibroblast Growth Factor-19, Recombinant, Human (FGF19, FGFJ)
Biozol Catalog Number:
USB-F4212-91A
Supplier Catalog Number:
F4212-91A
Alternative Catalog Number:
USB-F4212-91A-25
Manufacturer:
US Biological
Category:
Molekularbiologie
The FGF family plays central roles during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-19, a member of the FGF family, is a high-affinity heparin-dependent ligand for FGFR4. FGF-19 is expressed during brain development and embryogenesis. Recombinant Human FGF-19 is a 21.8kD protein containing 195 amino acid residues. Recombinant protein corresponding to 195aa from human Fibroblast Growth Factor 19 expressed in E. coli. Molecular Weight: ~21.8kD Amino Acid Sequence: MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK Biological Activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 for this effect is 100-150ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1-1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
