Fibronectin is a high molecular weight dimeric glycoprotein which is present in soluble and insoluble forms. The soluble form is the major component of blood plasma and is mainly produced by hepatocytes while insoluble fibronectin is a major component of the extracellular matrix. Fibronectin is involved in cell adhesion, growth, migration, and differentiation. Source: Purified fibronectin from goat plasma. Applications: Suitable for use in SDS-PAGE. Other applications not tested. Reconstitution: The purified protein is supplied in lyophilized form and may be dissolved in 4M urea solution, or dissolved in Cyclohexylaminopropanesulfonic Acid (CAPS) buffer at pH 11. Once dissolved, diluting from this buffer to a neutral pH will maintain active immunological and biological functions. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile urea. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
50
Purity:
Purified by affinity chromatography on gelatin covalently linked to agarose. 90% (SDS-PAGE).
Form:
Supplied as a lyophilized powder from 0.1M ammonium bicarbonate.
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