FIMC, Recombinant, E. coli, aa37-241, His-Tag (Chaperone Protein fimC, fimC Chaperone Periplasmic, b4316, ECK4307, JW4279)
Biozol Catalog Number:
USB-F4512
Supplier Catalog Number:
F4512
Alternative Catalog Number:
USB-F4512-100,USB-F4512-500
Manufacturer:
US Biological
Category:
Molekularbiologie
The 23kD two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Source: Recombinant corresponding to human FIMC, fused to His-tag at N-terminus, expressed in E. coli. Molecular Weight: ~25kD Sequences of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
