Recombinant protein corresponding to Arg25-Met233 of human FOLR1, expressed in CHO cells. Species Sequence Homology: mouse and rat, 83%.
FOLR1 is a 37-42kD protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine and histidine breakdown. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up- regulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml Flow Cytometry: 0.25ug labels/10e6 cells. Tested in MCF-7 cells. Immunocytochemistry: 8-25ug/ml. Tested on immersion fixed MCF-7 cells. Western Blot: 2ug/ml detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only. Optimal dilutions to be determined by the researcher. Matched Pair: Capture: F5750-16 Detection: 378687 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
