GADD45A, Recombinant, Human (Growth Arrest and DNA Damage-inducible Protein GADD45 alpha, DNA Damage-inducible Transcript 1 Protein, DDIT1, DDIT-1, GADD45)
GADD45A, Recombinant, Human (Growth Arrest and DNA Damage-inducible Protein GADD45 alpha, DNA Damage-inducible Transcript 1 Protein, DDIT1, DDIT-1, GADD45)
GADD45A, Recombinant, Human (Growth Arrest and DNA Damage-inducible Protein GADD45 alpha, DNA Damage-inducible Transcript 1 Protein, DDIT1, DDIT-1, GADD45)
GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E. coli. Recombinant corresponding to aa1-165 of human GADD45A 6x His tagged expressed in E. coli. AA Sequence: MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
