GCDH, aa45-438, Recombinant, Human (ACAD5, GCD, Glutaryl-CoA dehydrogenase, mitochondrial)

Product Overview

• Article Name: GCDH, aa45-438, Recombinant, Human (ACAD5, GCD, Glutaryl-CoA dehydrogenase, mitochondrial)
• Biozol Catalog Number: USB-G2023-32
• Supplier Catalog Number: G2023-32
• Alternative Catalog Number: USB-G2023-32-50,USB-G2023-32-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Glutaryl-CoA dehydrogenase(GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Source: Recombinant corresponding to aa45-438 of human GCDH 6x his tagged, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV SSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 45.8
• Purity: ~90% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 5mM DTT, 200mM sodium chloride.