GLUT 2, Rat (Glucose Transporter, Insulin Regulatable) (Control Peptide)

Product Overview

• Article Name: GLUT 2, Rat (Glucose Transporter, Insulin Regulatable) (Control Peptide)
• Biozol Catalog Number: USB-G3900-15F
• Supplier Catalog Number: G3900-15F
• Alternative Catalog Number: USB-G3900-15F-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Control Peptide for G3900-15D (affinity purified antibody) and G3900-15 (antiserum). G3900-15F is 16 amino acid peptide sequence corresponding to the exoplasmic loop between helices 1 and 2 of rat liver Glucose Transporter. Rat GT21 peptide sequence has 93% identity with mouse and human Glut-2 (15/16 AA). Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, 40-60kD) and sodium glucose co-transporter-1 (SGLT-1, 662 amino acids, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier, and to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons. Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1, ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2, ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa, , monkey 621aa,~ 50 kD, ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue and small intestine. Glut-13 or proton myo-inositol transporter (HMIT, human 629aa, rat 618aa, ~75-90 kD/67 kD protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol. Rat HMIT is ~35% identical to rat GlutX1. Applications: Suitable for use in ELISA, Antibody Blocking. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml. Antibody Blocking: 5-10ug per 1ug G3900-15D (affinity purified antibody) or per 1ul G3900-15 (antiserum). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Highly purified
• Form: Supplied as a liquid in PBS, pH 7.2