GLUT 10, Control Peptide, Human (Glucose Transporter)

Product Overview

• Article Name: GLUT 10, Control Peptide, Human (Glucose Transporter)
• Biozol Catalog Number: USB-G3900-95
• Supplier Catalog Number: G3900-95
• Alternative Catalog Number: USB-G3900-95-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA

Product Description

Control Peptide for G3900-95A (antiserum) and G3900-95D (affinity purified) A 16aa peptide sequence near the cytoplasmic, C-terminus of Human Glut-11 (1) was coupled to KLH. Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, MW. 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and c-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudoegene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541 aa, chromosome 20q13.1, ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496 aa, chromosome 22q11.2, ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Dilution: ELISA: Control peptide can be use to coat plates at 5-10ug/ml and detected with 1ul G3900-95A (antiserum) and 1ug G3900-95D (affinity purified). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Form: Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.