Glutamate Transporter EAAT2, Control Peptide, Rat (GLT1)
Biozol Catalog Number:
USB-G4005-91A
Supplier Catalog Number:
G4005-91A
Alternative Catalog Number:
USB-G4005-91A-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control Peptide for G4005-91 (affinity purified) and G4005-91B (antiserum). Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4 and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamate transporters (525-573aa) display about 55% homology and are predicted to contain up to 6-10 transmembrane domains. Immunolocalization studies indicate that GLT1 is localized in astroglial cells throughout the brain and spinal cord. EAAC1 is specific for certain neurons and Purkinje cells. It is specifically enriched in cortex, hippocampus and caudate-putamen. it is confined to presynaptic and postsynaptic elements. GLAST has been observed in both neuron and astroglia. It is most abundant in Bergmann glia, cortex, hippocampus and cerebellum. EAAT4 has properties of ligand gated Cl-channel. It is localized mainly in cerebellar Purkinje cells in rat and human CNS. EAAT5 has only been cloned from human. It is primarily expressed in retina. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. RecommendedDilution: ELISA: Coat plate at 1ug/ml. Antibody Blocking: 5-10ug per 1ug affinity pure IgG (G4005-91) or 1ul antiserum (G4005-91B). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
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