Glutamine Assay Kit, BioAssay(TM)

Product Overview

• Article Name: Glutamine Assay Kit, BioAssay(TM)
• Biozol Catalog Number: USB-G7120-05
• Supplier Catalog Number: G7120-05
• Alternative Catalog Number: USB-G7120-05-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Glutamine is an amino acid synthesized in the muscle that plays major roles in protein synthesis, acid-base balance, anabolic processes and is utilized for cellular energy and as a carbon source. It is used in treatment of injury, trauma, burns, and also as a supplement for muscle growth and post-surgery healing. Simple, direct and automation-ready procedures for measuring glutamine concentration are very desirable. This Glutamine Assay Kit is based on hydrolysis of glutamine to glutamate and colorimetric determination of the product. The intensity of the product color, measured at 565nm, is proportional to the glutamine concentration in the sample. Key Features: Sensitive and accurate: Use 20ul sample. Linear detection range of 0.023-2mM glutamine in 96-well plate assay. Convenient: The procedure involves adding a single working reagent, incubation for 40 minutes at RT, adding a stop reagent and reading the optical density. No 37C heater is needed. High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Applications: Direct Assays: Glutamine in serum, plasma, urine, tissue extracts and cell culture samples. Drug Discovery/Pharmacology: Effects of drugs on glutamine metabolism. Kit Components: G7120-05A: Enzyme A, 1x120ul G7120-05B: Enzyme B, 1x220ul G7120-05C: Assay Buffer, 1x15ml G7120-05D: Standard, 100mM Glutamine, 1x400ul G7120-05E: NAD Solution, 1x1ml G7120-05F: MTT Solution, 2x1.5ml G7120-05G: Stop Reagent, 1x25ml Storage and Stability: Store all components at -20C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials prior to removing the cap.