Glutaredoxin-5, Recombinant, Human (Glutaredoxin 5, GLRX5, GRX5, C14orf87, FLB4739, Glutaredoxin-related Protein 5 Mitochondrial, Monothiol Glutaredoxin-5, PR01238, PRO1238, MGC14129)
Biozol Catalog Number:
USB-G7210-50
Supplier Catalog Number:
G7210-50
Alternative Catalog Number:
USB-G7210-50-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
GLRX5 is a small redox enzyme of approximately one hundred amino-acid residues which uses glutathione as a cofactor. This protein is oxidized by substrates, and reduced non-enzymatically by glutathione. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Recombinant human GLRX5 protein, fused to His-tag at N-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-157 of human GLRX5 6x His tagged expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
