Glutathione S-Transferase, Recombinant Schistosoma japonicum, His-Tag, aa1-218 (GST)
Catalog Number:
USB-G8135-12G
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| Article Name: | Glutathione S-Transferase, Recombinant Schistosoma japonicum, His-Tag, aa1-218 (GST) |
| Biozol Catalog Number: | USB-G8135-12G |
| Supplier Catalog Number: | G8135-12G |
| Alternative Catalog Number: | USB-G8135-12G-100 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E. coli source. Recombinant human GST, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa1-218 with a His-Tag from Schistosoma japonicum GST, expressed in E. coli. Accession: AAB59203.1 Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR Enzyme Activity: Specific activity is >26.12 units/mg and is defined as the amount of enzyme that conjugates 1umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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