Glutathione S-Transferase pi, Western Blot Positive Control (GSTpi)

Product Overview

• Article Name: Glutathione S-Transferase pi, Western Blot Positive Control (GSTpi)
• Biozol Catalog Number: USB-G8135-26D
• Supplier Catalog Number: G8135-26D
• Alternative Catalog Number: USB-G8135-26D-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: WB

Product Description

Control peptide for G8135-26H. GSTs are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y. GST Pi/ GST P1/ GST3, a 210aa protein (chrm 11q13) is present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. Its expression was increased in many tumors relative to matched normal tissue and abundantly expressed in human skin. The protein shares 86% sequence identity with GSTP. GST Pi show enzyme activity towards CDNB, acrolein, adenine propenal, BDPE, benzyl isothiocyante, EA, 4-vinylpyridine. Source: GST-pi, purified by HPLC from rat tissues to homogeneity. Molecular Weight: ~25kD Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 25
• Purity: Purified
• Form: Supplied as a liquid in SDS-PAGE sample buffer (reduced)