Glutathione S-transferase theta 1, Recombinant, Human (GSTT1)
Biozol Catalog Number:
USB-G8135-27Z
Supplier Catalog Number:
G8135-27Z
Alternative Catalog Number:
USB-G8135-27Z-100,USB-G8135-27Z-500
Manufacturer:
US Biological
Category:
Molekularbiologie
Glutathione S-transferase theta 1(GSTT1) is one of the GSTs four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e.g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Source: Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Molecular Weight: 31.5kD(277aa) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR. Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: For long-term storage, aliquot and store at -20C/-70C. Aliquots are stable for at least 6 months at -20C/-70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
31.5
Purity:
95% by SDS-PAGE.
Form:
Supplied as a liquid in 20mM Tris-HCl buffer ,pH 8.0, 10% glycerol.
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