Each vial contains sufficient enzyme for 2 assays using CDNB as substrate in a spectrophotometric assay. Source: Rat Liver Specific Activity: 5.9units/mg Applications: Suitable for use as a standard for Western blotting or for ELISAs. Other applications not tested. Optimum dilutions to be determined by researcher. Definition of unit: One unit is the amount of enzyme that catalyzes the conjugation of 1uM of 1-chloro-2,4-dinitrobenzene (CDNB) to glutathione in one minute at room temperature in 100mM potassium phosphate buffer, pH 6.5. [J.B.C., 249 : 7130-7139 (1974)] Storage and Stability: Stable indefinitely at -70C. Labile to repeated freezing and thawing.
Purity:
Homogeneous by SDS gel electrophoresis.
Form:
Suspended in 20mM Tris buffer (pH 7.0).
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