Glyceraldehyde 3-Phosphate Dehydrogenase, Rabbit, Western Blot Control (GAPD, GAPDH, G3PD, MGC88685)
Biozol Catalog Number:
USB-G8140-14A
Supplier Catalog Number:
G8140-14A
Alternative Catalog Number:
USB-G8140-14A-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
Glyceraldehyde-3-phosphate dehydrogenase,G3PDH/GAPD/G3PD (EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). G3PDH is a cytosolic enzyme. It is thought to be a tetramer of identical chains ~36kD. G3PDH (mouse/rat/rabbit 333, human 335-aa,chromosome 12p13.31-p13.1). The enzyme is present in such widely separated forms as man, lobster, and E. coli. Its rate of evolutionary change is one of the slowest known. Applications: Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilutions: Western Blot: Heat once and load 10ul/lane and visualize with G8140-14B. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
