Glyceraldehyde 3-Phosphate Dehydrogenase, Recombinant, Human (GAPD, GAPDH, G3PD, MGC88685)
Biozol Catalog Number:
USB-G8140-14D
Supplier Catalog Number:
G8140-14D
Alternative Catalog Number:
USB-G8140-14D-100,USB-G8140-14D-500
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kD subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein, 1-335aa, was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight: 36kD (335 aa) Sequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
