Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Form: Supplied as a lyophilized powder. from PBS, pH7.2-7.4 Reconstitute with buffer to a concentration of 1mg/ml. Activity: > 4ug/ml will agglutinate fresh A1 cells. Older B cells can react stronger than A2 cells. Carbohydrate Specificity: a and b N-acetylgalactosamine > a and b galactose. Inhibitory Carbohydrate: Terminal a and b N-acetylgalactosamine > galactose. Quality Control: SDS-PAGE Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile buffer, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight:
110
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