Glycogen is a highly purified polysaccharide from oysters and can be used as a carrier for nucleic acid precipitation thereby replacing tRNA, linear polyacrylamide or sonicated DNA. Glycogen is an inert material compatible with most molecular biology procedures: PCR*, DNA sequencing, restriction digestion, ligation, competent cell transformation, cDNA synthesis, DNA labeling by kinase reactions and random priming, in vitro transcription/translation, gel electrophoresis, etc. Glycogen is a branched biopolymer consisting of linear chains of glucose residues with further chains branching off every 8 to 12 glucoses or so. Glucoses are linked together linearly by alpha(14) glycosidic bonds from one glucose to the next. Branches are linked to the chains from which they are branching off by alpha(16) glycosidic bonds between the first glucose of the new branch and a glucose on the stem chain. Due to the way glycogen is synthesised, every glycogen granule has at its core a glycogenin protein. Purity (Glycogen): 75% dry basis Appearance: White to off-white powder Infrared Spectrum: Conforms Loss on Drying: 10% Reducing Sugars: 1% Storage and Stability: Lyophilized powder may be stored at 4C. Product is stable for 12 months at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number:
[9005-79-2]
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