Human GSK3alpha GenBank Accession No. BC027984), full length, with N-terminal GST tag, MW 81.5kD, expressed in an Baculovirus infected Sf9 cell expression system. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: 700 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of glycogen synthase peptide 2 substrate per minute at pH 7.4 and 30C Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3 mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP, 20uM glycogen synthase peptide 2 substrate, and 2ug/ml recombinant GSK3alpha. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
