GSKIP (GSK3 B interaction protein) is defined as a naturally occurring protein that is homologous with the GSK3beta interaction domain of Axin and is able to negatively regulate GSK3beta of the Wnt signaling pathway. GSKIP may affect GSK3B activity on neurite outgrowth by inhibiting the specific phosphorylation of tau (ser396). Source: Recombinant corresponding to aa1-139 from human GSKIP, fused to His-tag at N-terminus expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH METDCNPMEL SSMSGFEEGS ELNGFEGTDM KDMRLEAEAV VNDVLFAVNN MFVSKSLRCA DDVAYINVET KERNRYCLEL TEAGLKVVGY AFDQVDDHLQ TPYHETVYSL LDTLSPAYRE AFGNALLQRL EALKRDGQS Molecular Weight: ~17.8kD (159aa), confirmed by MALDI-TOF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
