Granzyme B, Recombinant, Mouse (C11, Cathespin G Like 1, CCP1, CGL1, CSPB, CTLA1,CTSGL1, Cytotoxic serine protease B, Cytotoxic T Lymphocyte Cytotoxic T lymphocyte proteinase 2, EC 3.4.21.79, Fragmentin 2, Granzyme 2, GRB, GZMB, H
Biozol Catalog Number:
USB-G8957-34
Supplier Catalog Number:
G8957-34
Alternative Catalog Number:
USB-G8957-34-2,USB-G8957-34-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor expressed in Baculovirus. The apparent molecular weight is 28.9kD by mass spectrometry. Biological Activity: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25C, is greater than 750 nM/min per ug of enzyme. Amino Acid Sequence: IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
