Green Fluorescent Protein, Recombinant, Aequorea victoria (Jellyfish), His-Tag
Biozol Catalog Number:
USB-G8965-10F
Supplier Catalog Number:
G8965-10F
Alternative Catalog Number:
USB-G8965-10F-200
Manufacturer:
US Biological
Category:
Molekularbiologie
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Source: Full length recombinant Aequorea victoria GFP, fused to C-terminal His-Tag, expressed in E. coli. Molecular Weight: ~28.6kD Absorbance: 395 and 470nm, emits green fluorescence at 509nm. Applications: Suitable for use as a quantitative control for GFP expression studies using GFP reporter vectors. It can also be used as a standardon protein gels and western immunoblots or for calibration of Fluorometers, flow cytometers and FACS machines. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
