Griffonia simplicifolia GS II (Bandeiraea simplicifolia) (Biotin)

Product Overview

• Article Name: Griffonia simplicifolia GS II (Bandeiraea simplicifolia) (Biotin)
• Biozol Catalog Number: USB-G8967-86B
• Supplier Catalog Number: G8967-86B
• Alternative Catalog Number: USB-G8967-86B-2
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA, IF, IHC, WB

Product Description

This lectin is a tetrameric glycoprotein composed of four subunits of nearly identical size. Each 30,000D subunit has a binding site for alpha- or beta-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively alpha- or beta-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200mM N-acetylglucosamine Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Form: Supplied as a lyophilized powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca++. Reconstitute with 1ml dH2O. Labeled with Biotin.