GSL I is a family of glycoproteins with molecular weights of ~114kD. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin. GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. Inhibiting Sugar: 500mM galactose or 100mM raffinose Applications: G8967-93 will agglutinate blood group B glutaraldehyde-fixed human red blood cells at a concentration of 2ug/ml. No agglutination of blood group A, glutaraldehyde-fixed, human red blood cells occurred at concentrations up to 0.1mg/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Homogeneous by SDS-PAGE
Form:
Supplied as a lyophilized powder. Reconstitute in 10mM HEPES, pH 8.5, 0.1mM Ca++. For preserving solutions, stored at 4C, 0.08% sodium azide can be used
* VAT and and shipping costs not included. Errors and price changes excepted
