GSL I-B4 isolectin contains only the B subunits and has been reported to be useful as a marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. Applications: Suitable for the detection of glycoproteins in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 5-20 ug/ml Immunohistochemistry (Paraffin or frozen sections): 5-20 ug/ml Western Blot: 5-20 ug/ml Optimal dilutions to be determined by the researcher. Sugar Specificity: Galactose Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in above buffer.
Form:
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM CaCl2. Labeled with Biotin.
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