GroEL, Recombinant, E. coli, aa1-548 (Chaperonin 60, cpn60, Heat Shock Protein 60, HSP60)

Product Overview

• Article Name: GroEL, Recombinant, E. coli, aa1-548 (Chaperonin 60, cpn60, Heat Shock Protein 60, HSP60)
• Biozol Catalog Number: USB-G8976-01
• Supplier Catalog Number: G8976-01
• Alternative Catalog Number: USB-G8976-01-100,USB-G8976-01-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

GroEL protein is the major heat shock protein of E. coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E. coli and cloned into an expression vector. Recombinant protein corresponding to aa1-548 from E. coli GroEL, expressed in E.coli Molecular Weight: ~57.3kD (548 amino acids) Enzyme Activity: ATPase activity of this product has not been determined. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 57.3
• NCBI: 418567
• UniProt: P0A6F5
• Purity: 95% by SDS PAGE, Purified by using conventional chromatography techniques.
• Form: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 100mM sodium chloride, 5mM DTT, 10% glycerol.