Human, recombinant, full length with C-terminal His tag expressed in Sf9 cells. Applications: Suitable for use in enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Specific Activity: ~400pmol/min/ug Recommended Dilution: Assay Conditions: 50mM Tris-HCl, pH 8.0, 137mM sodium chloride, 2.7mM of KCl, 1mM MgCl2, 20uM substrate, 5ug BSA, H1827-53B. Incubate at 37C for 30 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
