Heat Shock Protein 27, Recombinant, Human, aa1-205 (HSP27)

Product Overview

• Article Name: Heat Shock Protein 27, Recombinant, Human, aa1-205 (HSP27)
• Biozol Catalog Number: USB-H1830-57H
• Supplier Catalog Number: H1830-57H
• Alternative Catalog Number: USB-H1830-57H-100,USB-H1830-57H-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38). Recombinant protein corresponding to aa1-205 from human Hsp27, overexpressed in E. coli. Swiss/Uniprot Accession: P04792 Molecular Weight: ~22.7kD (205aa) confirmed by MALDI-TOF Amino Acid Sequence: MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 22.7
• NCBI: 001531
• UniProt: P04792
• Purity: 95% by SDS-PAGE. Purified by using conventional chromatography techniques. Endotoxin: 1EU/ug (LAL).
• Form: Supplied as a liquid in 20mM HEPES, pH 7.4, 1mM DTT, 100mM potassium chloride.